Fig 4. Trypsin inhibition assays.

(A) Baseline trypsin (0.3 μg/mL) activity with Nα-benzoyl-L-arginine ethyl ester (75 μM). Inhibition of trypsin with (B) Roche protease inhibitor cocktail (60 μg/mL) and (C) TFP4 (2 μg/mL). Additions are indicated by arrows. The hydrolysis rate and correlation coefficient (R²) are shown for each reaction. The assay with crude extract was not performed since no inhibition was observed with the recombinant protein.