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. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: Alcohol Clin Exp Res. 2014 Feb 11;38(5):1356–1364. doi: 10.1111/acer.12358

Fig. 1.

Fig. 1

Chronic EtOH feeding reduces baseline numbers of dendritic epidermal T cells (DETCs). Fresh epidermal cell preparations from ear skin of mice fed EtOH for 12 to 16 weeks or age-matched water controls were counted and stained for flow cytometry. (A) Gating strategy for identifying epidermal LCs and DETCs from a representative H2O control mouse. (B) Numbers and frequencies of LCs and DETCs/ear. Error bars represent SEM. ***Post test p-value < 0.0005. n ≥ 22 mice/treatment group. EtOH, ethanol; LCs, Langerhans cells.