Skip to main content
. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: Alcohol Clin Exp Res. 2014 Feb 11;38(5):1356–1364. doi: 10.1111/acer.12358

Fig. 2.

Fig. 2

Chronic EtOH feeding reduces baseline numbers of dermal γδ T cells. Fresh dermal cell preparations from ear skin of mice fed EtOH for 12 to 16 weeks or age-matched water controls were counted and stained for flow cytometry. (A) Gating strategy for dermal T cell subsets for a representative H2O control mouse. (B) Numbers of dermal T cells/ear. Total γδ T cells were calculated as the sum of the CD3hi-gated cells and the γδ+ fraction within the CD3+ CD103+ gate. Likewise, total αβ T cells were calculated as the sum of CD3+ CD103− gated cells and the γδ-fraction within the CD3+ CD103+ gate. Error bars represent SEM. *Post test p-value < 0.05. n ≥ 8 mice/treatment group. EtOH, ethanol.