Skip to main content
. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: Alcohol Clin Exp Res. 2014 Feb 11;38(5):1356–1364. doi: 10.1111/acer.12358

Fig. 3.

Fig. 3

Chronic EtOH feeding reduces baseline numbers of dermal Tregs. Fresh dermal cell preparations from ear skin of mice fed EtOH for 12 to 16 weeks or age-matched water controls for both WT and FoxP3-GFP mice were counted and stained for flow cytometry. (A) Gating strategy for dermal Treg identification in both Foxp3-GFP and WT mice for representative H2O control mice. (B) Numbers of dermal Tregs/ear. Error bars represent SEM. *Post test p-value < 0.05. n ≥ 6 mice/treatment group. EtOH, ethanol; Treg, regulatory T cell; WT, wild-type.