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. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: Alcohol Clin Exp Res. 2014 Feb 11;38(5):1356–1364. doi: 10.1111/acer.12358

Fig. 7.

Fig. 7

Chronic EtOH feeding reduces IL-17A production by lymph node (LN) γδ T cells, but not TH17 cells. LN cell suspensions prepared from mice fed EtOH for 12 to 16 weeks or age-matched water controls were cultured 4 hours − stimulation with plate-bound anti-CD3 or phorbol 12-myristate 13-acetate (PMA) and ionomycin. IL-17A-producing αβ or γδ T cells present in cultures prepared from EtOH-fed or control mice were identified according to gating strategy shown in the dot plot. Error bars represent SEM. *Post test p-value < 0.05. n ≥ 8 mice/treatment group. EtOH, ethanol; IL-17A, interleukin 17A.