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. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: J Immunol. 2013 Jun 17;191(2):865–874. doi: 10.4049/jimmunol.1201648

Figure 2. HBD2 binds self DNA and induces IFN production by pDCs.

Figure 2

(A) Human genomic DNA labeled with Alexa 488 (5μg) was mixed with HBD2 (1μM) for 30 minutes at RT and added to pDC cultures. Cells were analyzed by FACS after 30 minutes. One representative experiment out of five is shown. (B) For IFN-α measurements HBD2 (2μM) was mixed with genomic DNA from fetal skin (10μg/ml) for 30 minutes. Resulting complexes were added to pDC cultures and supernatants were collected after 18-24 hrs and IFN-α was measured. Statistical significance was determined using Student’s t-test. Error bars indicate SEM. One representative experiment out of five is shown. ** P <0.001