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. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: J Immunol. 2013 Jun 17;191(2):865–874. doi: 10.4049/jimmunol.1201648

Figure 3. HBD3-DNA mediated IFN-α production is dependent on TLR9.

Figure 3

(A) IFN A4 promoter activity in HEK cells transfected with IRF7/TLR4 or IRF/TLR9 after treatment with HBD3/DNA complexes. CpG/DoTap was used as positive control. One representative experiment out of two is shown. Error bars indicate SEM. * P<0.05, ** P <0.01. (B) pDCs were pretreated with either ODN TTAGGG or control ODN (10:1 ODN/DNA ratio) before adding HBD3-DNA complexes. One representative experiment out of two is shown. (C) pDCs were treated with indicated alarmins in the presence or absence of DNA. IFN-α levels were measured after 24 hrs. One representative experiment out of two is shown. Error bars indicate SEM. (ANOVA one-way analysis of variance)* P<0.05** P <0.001