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. Author manuscript; available in PMC: 2015 May 15.
Published in final edited form as: J Immunol. 2013 Jun 17;191(2):865–874. doi: 10.4049/jimmunol.1201648

Figure 6. Isothermal titration calorimetry (ITC) experiment with DNA CpG ODN35 binding to protein HBD3.

Figure 6

The exothermic binding isotherm (above) was integrated to give the enthalpy change plotted as a function of the molar ratio of DNA/protein (panel A) or protein/DNA (Panel B). The integrated enthalpy change data were used to calculate the binding affinity. One representative experiment out of two is shown.