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. 2015 May 15;11(5):e1005232. doi: 10.1371/journal.pgen.1005232

Fig 3. ChIP-Seq analysis reveals direct targets of CtrA.

Fig 3

Genes directly regulated by CtrA. A. Representation of all CtrA binding sites in the three circular replicons of S. meliloti (here represented as linear starting from the origin of replication). B. Promoter region of several genes detected by microarrays. Transcriptional start sites, previously defined [39], are represented as orange arrows (numbers between brackets represent the distance form ATG). In blue the plot of reads per nucleotide measured by ChIP-Seq analysis in a 600bp long region including the beginning of the coding sequence (in red). Green lines represent predicted CtrA binding site [11]. C. ChIP-Seq of the ccrM promoter region. In blue the plot of reads per nucleotide measured by ChIP-Seq analysis. Green lines represent predicted CtrA binding site. D. Beta-galactosidase activity assay using a LacZ fusion of the ccrM promoter in cells (BM249) after depletion of CtrA (no IPTG) for two hours relative to control cells expressing CtrA (+IPTG). Error bars indicate standard error.