Fig 2. Photoadaptation defect exists at low light intensity and mutations do not effect nuclear localization of VVD.

(A) Strains (n = 3) were exposed (after 48 hours in dark) to very low light intensity blue light (~1.5 μM m^-2s^-1 of blue light) for 15 minutes and 60 minutes to study light response and photoadaptation via al-3 gene expression. (B) N-terminal GFP tagged VVD mutant strains were created for sub-localization studies and photoadaptation defect was tested after 60 minute bright white light(40 μM m^-2s^-1) exposure in these strains using al-3 expression as readout (n = 3). (C) Fixed cell microscopy qualitatively shows that the mutants retain the ability to localize VVD protein to the nucleus after exposure to white light (40 μM m^-2s^-1) induces expression. Asterisks indicate statistical significance as determined by an unpaired t test. *P<0.05, **P<0.01, ***P < 0.001.