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. 2015 May 15;10(5):e0126726. doi: 10.1371/journal.pone.0126726

Fig 6. CRAds-induced autophagy in TERT+EphA3+ tumor cells.

Fig 6

At 40 h postinfection (1–10 MOI) with CRAds, C4-2B cell autophagy was measured. A, B, C: autophagosomes were stained with arcridine orange and observed microscopically. Autophagosomes are red vesicles in the cytoplasm. Bar = 40 Am. Autophagy increased with increasing infective dose. Autophagy caused by Ad-TERTp-E1A-1504 (1, 5, and 10 MOI) exceeded that of Ad-TERTp-E1A-NC at the same MOI. Autophagy of CRAds at 5 and 10 MOI exceeded that of control and autophagy at 10 MOI were greater than that at 5 MOI. D: Beclin1 and p62 protein was measured with Western blot after treatment with Ad-TERTp-E1A-1504 and Ad-TERTp-E1A-NC.