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. 2015 May 15;10(5):e0125427. doi: 10.1371/journal.pone.0125427

Fig 2. Expression of Brassica napus precursor miR394a/b and its target BnLCR in wild type (WT) and the transgenic plants.

Fig 2

Total RNA from two week-old seedlings was extracted and transcripts were analyzed. A: transcripts of pre-miR394a/b and BnLCR were analyzed using semi-quantitative RT-PCR. B: Base paring of miR394 with the corresponding complementary site of BnLCR and a miR394-resistant form of BnLCR (Bnm6LCR). C: Transcripts of BnLCR in miR394-resistant (35S::Bnm6LCR) and miR394 non-resistant (35S::BnLCR) transgenic lines. Actin was used for cDNA normalization. The number below the band indicates relative abundance of the corresponding gene with respect to the loading controls Actin. or 35S:m5LCR plants and WT (p < 0.05).