Fig 1. Zebrafish embryo-larval survival analysis following escalating doses of nicotine, e-cigarette and tobacco extracts.

Assessment of zebrafish survival over 72 h with increasing concentrations of pure nicotine, e-cigarettes and tobacco cigarettes extracts or vehicle control (0.1% DMSO). n ≥ 3 (independent experiments with each n containing between 24–48 animals per treatment). * P < 0.05, hpe = hours post exposure.