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. 2015 May 15;10(5):e0126259. doi: 10.1371/journal.pone.0126259

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© 2015 Palpant et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — (a) Timeline of differentiation protocol for cardiac directed differentiation of hESCs. (b-e) Analysis of cardiac endpoints including intrinsic beating rate (b) cardiomyocyte yield (c), cardiomyocyte purity (d), and cardiomyocyte immaturity based on percent cTnT⁺/SMA⁺ (e) and representative flow cytometry plots (f) on day 14 of differentiation with increasing doses of purified e-cigarette and tobacco cigarette extracts. n ≥ 6 per group. * P < 0.05.