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. 2015 May 15;10(5):e0126795. doi: 10.1371/journal.pone.0126795

Fig 2. In vivo ATF4 reporter, tub-ATF4.5′UTR>dsRed responds to protein misfolding.

Fig 2

(A) The structure of the ATF4 translational activation reporter, tub-ATF4.5′UTR>dsRed. The dsRed ORF replaces that of ATF4, thereby reporting ATF4′s translation. (B-E) Validation of the reporter response in vivo. Control eye discs did not show dsRed expression (B). Misexpression of Aβ activated the tub-ATF4.5′UTR>dsRed reporter, as evidenced by the expression of dsRed (C). The ATF4 reporter pattern in response to Rh-1G69D misexpression in the eye disc (D). Endogenous ATF4 expression detected using anti-ATF4 antibody labeling (E).