Table 3. Primers and probe sets for QRT-PCR.

Viral RNA target	QPCR primers & probe	Sequence (5’ to 3’)
S vRNA	S 2275+	CGCTGGCCTGGGTGAAT
	S 2338-	ATGGGAAAACACAACAATTGATCTC
	S 2295+P a	FAM-CTGCAGGTTTCTCGC-MGBNFQ b
S vcRNA	S 929+	TTGCCTGACCAAATGGATGAT
	S 988-	ACTGCTGTGTTCCCGAAACAC
	S 952+P	FAM-TTGCTGCAGAGCTTA- MGBNFQ
L vRNA	L 5517+	GGCCTTGTATGGAGTAGCACCTT
	L 5645-	GGTCTGTGAGATATCAAGTGGTAGAATG
	L 5582-P	FAM-CTGAAGAATACCACCTATTATACCA- MGBNFQ
L vcRNA	L 212+	TTGGTAAGATGCCATGACCACTA
	L 276-	TCGGATACTGACAGCAGAAGGTT
	L 251-P	FAM-AACAGTGCCTGCAAAG- MGBNFQ

^aP, TaqMan probe.

^bMGBNFQ, minor groove binder and non-fluorescent quencher dye.