Table 4. Percent of primer-specific target RNA signal generated in the absence of an RT primer.
| Source of RNA | Time pi | Percent of target RNA a | |||
|---|---|---|---|---|---|
| S segment | L segment | ||||
| MC57 cells | vRNA | vcRNA | vRNA | vcRNA | |
| 12 hr | nd b | nd | 18.2 ± 0.5 | 23.1 ± 0.7 | |
| 18 hr | 7.4 ± 0.3 | 14.5 ± 0.1 | 16.1 ± 1.0 | 29.3 ± 0.9 | |
| 7 d | 8.1 ± 0.8 | 15.1 ± 0.8 | 18.9 ± 0.7 | 17.1 ± 0.1 | |
| 7 d | 8.8 ± 0.1 | 11.2 ± 0.1 | 14.3 ± 0.6 | 14.0 ± 0.1 | |
| 7 d | 8.4 ± 0.1 | 13.7 ± 0.6 | 9.7 ± 0.1 | 14.1 ± 0.2 | |
| 14 d | 9.7 ± 3.8 | 11.9 ± 0.1 | nd | nd | |
| 24 d | 6.7 ± 0.2 | 14.0 ± 0.5 | 16.0 ± 1.1 | 11.2 ± 0.3 | |
| Mouse spleen #1 | 4 d | 5.4 ± 4.9 | 6.8 ± 0.4 | 3.4 ± 0.3 | 44.8 ± 6.6 |
| Mouse spleen #2 | 4 d | 3.9 ± 0.8 | 5.3 ± 2.0 | 2.2 ± 2.3 | 58.4 ± 3.0 |
| Supernatant from MC57 cells | 12 hr | 4.0 ± 0.7 | 8.7 ± 0.9 | 14.7 ± 0.3 | 102.6 ± 11.4 |
| 18 hr | 5.0 ± 0.3 | 32.5 ± 2.2 | 21.2 ± 4.1 | 111.7 ± 12.9 | |
| 24 d | 6.9 ± 3.7 | 5.2 ± 1.4 | nd | nd | |
| Sucrose-banded virions from Vero E6 cells | 48 hr | 6.2 ± 2.6 | 111 ± 2.8 | 6.3 ± 0.7 | 73.1 ± 3.6 |
aFor each RNA species, the numerical values represent the mean copy number ± standard deviation obtained via QRT-PCR in the absence of an LCMV segment-specific primer during the RT step divided by the copy number obtained when the segment-specific primer was used during RT. Each value was multiplied by 100 to convert to percentage.
bnd, not done.