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Sensitization of saline intake in the water-first/saline-second protocol is not dependent on a decrease in water intake. Total 1.8% saline intake is displayed on the y-axis. When offered only 4 mls of water to drink during each test, rats displayed a progressive elevation of saline intake (*=p<0.05 vs. depletion 1, #=p<0.01 vs. depletion 1), similar to rats with unlimited water access. All data are expressed as mean ± SEM.
