Table 1.

Physicochemical properties and diffusivity of gene vectors in cortical tissue

	PEG/PEI w/w(a)	Hydrodynamic Diameter ± SEM [nm](b)	ζ-potential ± SEM [mV](b)	PDI(b)	Hydrodynamic Diameter in ACSF [nm](c)	MSDAQ/MSDBrain(d)
DNA-UPN	0	47 ± 2	26 ± 1.2	0.15	1071 ± 53	6900
DNA-CPN	1.6	59 ± 1	9.3 ± 0.5	0.17	193 ± 4	930
DNA-BPN	3.9	43 ± 5	2.9 ± 0.3	0.19	80 ± 9	260

^(a)PEG/PEI weight to weight ratio used to formulate respective NP.

^(b)Size, ζ-potential and polydispersity (PDI) were measured by dynamic light scattering (DLS) in 10 mM NaCl at pH 7.0 and are presented as an average of at least 3 measurements ± standard error of the mean (SEM).

^(c)Hydrodynamic diameter in aCSF was measured by DLS following incubation in aCSF at 37 °C for 1 h.

^(d)MSD at 1 s was measured using MPT of fluorescently labeled gene vectors in rodent brain slice. NP diffusivity in aCSF was calculated using the Stokes-Einstein equation and the mean particle diameter.