Table 1.
PEG/PEI w/w(a) | Hydrodynamic Diameter ± SEM [nm](b) | ζ-potential ± SEM [mV](b) | PDI(b) | Hydrodynamic Diameter in ACSF [nm](c) | MSDAQ/MSDBrain(d) | |
---|---|---|---|---|---|---|
DNA-UPN | 0 | 47 ± 2 | 26 ± 1.2 | 0.15 | 1071 ± 53 | 6900 |
DNA-CPN | 1.6 | 59 ± 1 | 9.3 ± 0.5 | 0.17 | 193 ± 4 | 930 |
DNA-BPN | 3.9 | 43 ± 5 | 2.9 ± 0.3 | 0.19 | 80 ± 9 | 260 |
PEG/PEI weight to weight ratio used to formulate respective NP.
Size, ζ-potential and polydispersity (PDI) were measured by dynamic light scattering (DLS) in 10 mM NaCl at pH 7.0 and are presented as an average of at least 3 measurements ± standard error of the mean (SEM).
Hydrodynamic diameter in aCSF was measured by DLS following incubation in aCSF at 37 °C for 1 h.
MSD at 1 s was measured using MPT of fluorescently labeled gene vectors in rodent brain slice. NP diffusivity in aCSF was calculated using the Stokes-Einstein equation and the mean particle diameter.