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. Author manuscript; available in PMC: 2016 May 13.
Published in final edited form as: Cell Host Microbe. 2015 Apr 16;17(5):681–689. doi: 10.1016/j.chom.2015.03.006

Figure 2. Diurnal gut microbe community structure is altered by high fat feeding.

Figure 2

Principal Coordinate Analysis (PCoA) of weighted UniFrac distances from 16S rRNA amplicon sequences colored by diet in fecal pellets (A) collected via repeat sampling over 48h (n=3 mice/treatment) and in cecal contents (B) collected over 24h (n=2–3 mice/time point) from SPF C57Bl/6 mice fed HF or RC. (C) PCoA analysis of cecal contents colored by Zeitgeber (ZT) time (PC=principle coordinate). (D) 16S rRNA abundance determined via qPCR using universal primers in cecal contents. Data represent mean ± s.e.m. **p<0.01; *p<0.05 via unpaired t-test at each time point. (E) Relative abundance of oscillating 16S rRNA OTUs determined via eJTK_CYCLE in fecal samples collected every 6h over 48h and in cecal contents (F) collected every 4h over 24h at sacrifice. Data represent mean ± s.e.m. See also Figure S2A–E, Table S3 and S5.