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. Author manuscript; available in PMC: 2015 Nov 14.
Published in final edited form as: Nature. 2015 Mar 2;521(7551):213–216. doi: 10.1038/nature14243

Figure 2. Protease-mediated defense responses are coupled to G protein signaling.

Figure 2

a, Western blot depicting activation of MAPKs by PrpL, ArgC or flg22 in wild-type Col-0 or G protein T-DNA mutants. The same molecular weight region of the blot is shown as in Fig. 1b. b, Induction of defense-related gene expression by PrpL, ArgC or flg22 in wild-type Col-0 or G protein T-DNA mutants measured by RT-qPCR. c, Protection of 4-week old wild-type Col-0 or gαβ double mutant leaves from P. syringae pv. tomato strain DC3000 infection by pre-infiltrated PrpL or flg22. gpa1-4 is a gα mutant; agb1-2 is a gβ mutant; and gpa1-4/agb1-2 is a gαβ double mutant. The data represent the mean ± s.d., n = 3 biological replicates with each experiment containing eight seedlings (b), and n = 10 leaves from 5 plants (c); *P < 0.05; **P < 0.01; ***P < 0.001, Student’s t-test vs. Col-0 (b) and vs. mock (c).