Extended Data Figure 4. Protease IV-triggered responses are dependent on proteolytic activity.

a, Induction of defense-related genes by 20 nM purified PrpL or inactive variants of PrpL measured by RT-qPCR. b, Induction of defense-related genes by 20 nM purified PrpL or 20 nM flg22, or 20 nM TLCK-treated PrpL or 20 nM TLCK-treated flg22 measured by RT-qPCR. c, Induction of defense-related genes by 20 nM PrpL or 20 nM heat-treated PrpL or 20 nM flg22 or 20 nM heat-treated flg22 measured by RT-qPCR. d, Chemiluminescence assay showing elicitation of an oxidative burst by 20 nM purified PrpL, 20 nM inactive variants of PrpL, or 20 nM TLCK-treated PrpL. The data represent the mean ± s.d., n = 3 biological replicates with each experiment contains eight seedlings (a, b, c), and n = 16 individual seedlings (d).