Skip to main content
. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Mol Cancer Res. 2015 Jan 30;13(5):819–827. doi: 10.1158/1541-7786.MCR-14-0492

Figure 2. Immunoblot analysis of purified OPG-mutants.

Figure 2

Forty-eight hours following transfection of expression plasmids containing the sequences of OPG-wt and OPG-mutants in HEK-293 cells, supernatant media were collected and concentrated using a 30 kDa filter. The concentrated media were then separated in a SDS-PAGE and transferred to nitrocellulose membrane. Loading control was assessed by Coomassie Blue staining (A) and detection of OPG-wt and OPG-mutants was performed using antibodies for IgG (B) and OPG (C).