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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Mol Cancer Res. 2015 Jan 30;13(5):819–827. doi: 10.1158/1541-7786.MCR-14-0492

Figure 3. Osteoclast assay to determine retention of RANKL binding of OPG-mutants.

Figure 3

Primary macrophage cells were cultured in the presence of 200 ng of recombinant OPG, OPG-wt or indicated OPG-mutants in the presence or absence of 60 ng of recombinant RANKL. As a positive control, macrophage cells were cultured with RANKL alone. Tartrate-resistant acid phosphatase (TRAP) staining of macrophage cell cultures were performed 7–14 days after cells were cultured in the presence of OPG-wt or OPG-mutants and RANKL (A). Quantitative analysis based on the number osteoclast seen in 10 random fields (B) (*p<0.005 compared to culture with RANKL only).