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. Author manuscript; available in PMC: 2016 May 13.
Published in final edited form as: Cell Host Microbe. 2015 Apr 16;17(5):628–641. doi: 10.1016/j.chom.2015.03.007

Figure 5. Coordinate α-OX40 ligation and PD-1 blockade during experimental malaria abrogates T follicular helper and germinal center B cell responses.

Figure 5

(A–E) Mice were infected with 106 P. yoelii-infected RBCs and treated with rat IgG, α-PD-L1, α-OX40, or α-PD-L1 and α-OX40 on days 7 and 10 p.i. (A) Parasitemia kinetics (n=5 mice/group). Data (mean ± SEM) in (A) are representative of 5 independent experiments. (B) Representative dot plots showing PD1+CXCR5+ Tfh cells and histograms showing Bcl-6 expression on days 14 (left) and 20 (right) p.i. (C) Cumulative data (mean ± SD) showing the total number of Bcl-6+ effector CD4 T cells on day 20 p.i. pooled from 2 independent experiments. (D) Representative dot plots showing CD19+GL-7+ B cells and histograms showing Bcl-6 expression on days 14 (left) and 20 (right) p.i. (E) Cumulative data (mean ± SD) showing the total number of Bcl-6+CD19+GL-7+ B cells on 20 p.i. pooled from 3 independent experiments were analyzed using one-way ANOVA while correcting for multiple comparisons via the Tukey method. Data in (B–E) are representative of 3 independent experiments. Symbols in (C,E) represent individual mice. See also Figure S5.