Skip to main content
. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Mol Cancer Res. 2015 Feb 12;13(5):934–943. doi: 10.1158/1541-7786.MCR-14-0604

Figure 2. Knockdown of MAPK7 induces Mesenchymal-Epithelial Transition (MET).

Figure 2

(A) MET is shown by qRT-PCR upon knockdown of shRNA against non-target (sh-NT) and two independent shRNAs against MAPK7 (sh-MAPK7-1 and sh-MAPK7-2) in A549 cells. Error bars represent SD from three individual experiments. * represents p-value < .05, ** represents p-value < .01 and *** represents p-value < .001.

(B) Knockdown of MAPK7 induces MET. Knockdown of MAPK7 in A549 cells increase CDH1 expression as demonstrated by immunoblot analysis. β-actin is used as loading control.

(C) Knockdown of MAPK7 induces CDH1 expression in A549 cells, demonstrated by immunofluorescence staining. Green = CDH1, red = VIM (Vimentin) and blue = dapi. Scale bar (white) = 50 μm.

(D) Knockdown of MAPK7 in A549 cells reduces cell migration as assayed using a Boyden Chamber. Quantitation of cell migration is derived from five independent fields. Error bars represent SD from three independent experiments.