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. Author manuscript; available in PMC: 2016 May 1.
Published in final edited form as: Mol Cancer Res. 2015 Feb 12;13(5):934–943. doi: 10.1158/1541-7786.MCR-14-0604

Figure 3. MAPK7 activates Snai2.

Figure 3

(A) Knockdown of MAPK7 in A549 cells decreases Snai2 expression whereas it has no effect on Snai1 and Zeb1 expression by qRT-PCR. Error bars represent SD from three individual experiments. * represents p-value < .05 and ** represents p-value < .01.

(B) Constitutive active MAPK7 (Myr-MAPK7) increases Snai2 expression, whereas a dominant negative MAPK7 mutant (DN-MAPK7) reduces Snai2 expression in A549 cells as shown by qRT-PCR. Error bars represent SD from three individual experiments. * represents p-value < .05, ** represents p-value < .01 and *** represents p-value < .001.

(C) Smartpool RNAi knockdown (Dharmacon) of Snai2 decreases CDH1 and increases SERPINE1 expression in A549 cells by qRT-PCR. GAPDH serves as knockdown control. Error bars represent SD from three individual experiments. * represents p-value < .05, ** represents p-value < .01 and *** represents p-value < .001.