Fig 2. Effect of the different yopD mutations on translocation.

A. yopD variants were expressed ectopically in a yopD single mutant strain and tested for YopE-mediated cell rounding of HeLa cells. Bacteria cultured under conditions that repress the T3SS were used to infect HeLa cells at MOI of 100 for 1h. YopE-mediated cytotoxicity was assessed by inverted microscopy using a 40× objective. Images were recorded using a digital camera. Images are representative of three different experiments.