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. 2015 Mar 25;10(3):e0120471. doi: 10.1371/journal.pone.0120471

Fig 3. Effect of the different YopD mutations on pore formation.

Fig 3

A. HeLa cells were left uninfected or infected with yopEHJD, yopEHJD/pYopD and yopEHJD expressing the different yopD mutants at MOI of 100. After 3 h infection, culture supernatants were removed and tested for LDH release. Background LDH released from uninfected cells was subtracted from infected wells. Results were normalized to yopEHJD/pYopD (100%). Error bars represent the standard deviation of the mean values obtained from four duplicate experiments. ** P<0.0001, * P<0.0005 determined by t-test. B. HeLa cells on coverslips were infected as described above for the LDH assay, and stained with DEAD-LIVE kit as described in Material and Methods. Cells with disrupted membranes exhibit a red nuclei staining.