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. 2015 Mar 25;10(3):e0120471. doi: 10.1371/journal.pone.0120471

Fig 4. YopD-dependent activation of proinflammatory signaling.

Fig 4

A. HeLa cells infected with yopEHJD, yopEHJD/pYopD and yopEHJD expressing the different YopD mutants at MOI of 100 for 1h. Cells were washed, lysed with sample buffer 1X, separated by SDS-PAGE and analyzed by immunoblotting with rabbit anti-phospho ERK. Monoclonal antibody against tubulin was used as a loading control. Quantification of the signal intensities was performed using Odyssey imaging system software. Values were normalized to tubulin.