Fig 7. Residues I168, G196 and A273 are not required for virulence of Y. pseudotuberculosis IP2666 in a mouse model.
Eight-week old C57BL/6J mice were infected orogastrically with 2×109 CFU of wild type Y. pseudotuberculosis IP2666 and its derivative expressing yopD I168T, G196R and A273T. Mouse survival was monitored for 14 days. Results shown are pooled from two independent experiments using 3 mice per group in each experiment. Statistical analysis using log rank test showed no significant differences between the survival curves of each of the mutants and that of the wild type.