Table 1. Bacterial strains and plasmids.

Strain	Relevant features	Reference
YP126	Wild type Y. pseudotuberculosis YPIII, naturally YopT-deficient	[46]
IP2666	Wild type Y. pseudotuberculosis YPIII, naturally YopT-deficient	[33]
YP18	YP126ΔyopB	[46]
YP27	yopH::cam,yopE::kan,ΔyopJ	[47]
YP29	yopH::cam,yopE::kan,ΔyopJ,ΔyopB	[47]
YP52	yopH::cam,yopE::kan,ΔyopJ ΔyopD	This study
YP52/pYopD	YP52::pYopD	This study
YP6	yopE::kan	[48]
YP62	yopE::kan,ΔyopD	This study
YP62/pYopD	YP62::pYopD	This study
YP63	YP126ΔyopD	This study
YP63/pYopD	YP63::pYopD	This study
IP63	IP2666ΔyopD	This study
pMMB67HE	IPTG-inducible expression	[49]
pMMB67HEYopD	pMMB67HE::yopD	[26]
pSB890	Suicide plasmid TetR, sacB	[46]
pSB890ΔyopD	pSB890::ΔyopD (177bp upstr yopD fused to 217bp downstr yopD)	This study
pSB890yopD AD	pSB890::yopD (−177bp yopD + 217bp)	This study