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Experimental and simulated response of the nanoslit biosensor at the single-pixel level (size: 1.3 × 1.3 μm). The normalized measured fluorescence is compared to the fraction of hybridized probe molecules (target concentrations: 100 nM), with an optimal fitting value of kon = 4 × 106 M−1s−1, is in agreement with a previous report39 (the error bars: standard errors).
