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. Author manuscript; available in PMC: 2016 Mar 1.
Published in final edited form as: J Cell Physiol. 2015 Mar;230(3):578–586. doi: 10.1002/jcp.24774

Figure 3.

Figure 3

OBs were cultured alone or in the presence of MKs for 5 or 6 days. MKs were removed by washing and OBs were lysed and resolved by SDS-PAGE. Western blotting shows a significant reduction in Mdm2, p53 and Rb protein levels in OBs co-cultured with MKs as compared to OBs cultured alone. It should be noted that the lanes in this blot represent cell lysates from 3 separate experiments (2 at day 5 and 1 at day 6). In all experiments conducted (more than 10 to date) with 4–6 days of culture, co-culture with MKs always results in a marked reduction in Mdm2, p53, and Rb protein levels in OBs.