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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: J Neurovirol. 2015 Feb 26;21(3):310–321. doi: 10.1007/s13365-014-0308-9

Figure 2. Precise cleavage of the third nucleotide from protospacer adjacent motif (PAM) by RNA-guided Cas9 dual nucleases induces double strand breaks in the target DNA.

Figure 2

The guide RNA (gRNA) is a chimeric stem-loop structure consisting of CRISPR RNA (crRNA) and trans-activating crRNA (tracRNA) with a GAAA tetraloop.