Figure 1. Inhibition of PI3K signaling decreased junctional conductance mediated by Cx50 but not Cx46.
(A) Gap junctional conductance between HeLa cell pairs expressing Cx50 treated with an inhibitor of the p110α catalytic subunit of PI3K (PIK-75), Akt inhibitor VIII (Akti), or the vehicle (DMSO). Cell pairs expressing Cx50 showed a three-fold decrease in gap junctional coupling after treatment with either PIK-75, or Akti. Treatment with vehicle (DMSO) had no effect. (B) HeLa cells transiently transfected with Cx46 and treated with PIK-75 showed no significant difference in gap junctional coupling compared to controls. Data are the mean ± SE.