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. Author manuscript; available in PMC: 2016 May 22.
Published in final edited form as: FEBS Lett. 2015 Apr 29;589(12):1340–1345. doi: 10.1016/j.febslet.2015.04.029

Figure 2. Treatment of Cx50 expressing HeLa cells with either a PI3K or an Akt inhibitor decreased levels of activated Akt protein.

Figure 2

Stably transfected Cx50 HeLa cells were treated with PIK-75, Akti, or vehicle for 24 hours then lysed in SDS buffer and ran on SDS-PAGE gels. Proteins were transferred to nitrocellulose membrane and probed for p-Akt and t-Akt. Band density measurements were averages of three independent experiments. (A) PIK-75 and Akti had no effect on total Akt. (B) Both PIK-75 and Akti decreased Akt phosphorylation. DMSO was used as a vehicle for the inhibitor, and had no effect on either p-Akt or t-Akt.