Figure 5. PI3K signaling inhibition did not reduce Cx50 single channel conductance.

HeLa cells stably expressing Cx50 were treated with either Akti or PIK-75 for 24 hours. Junctional currents were measured by bipolar V_j pulses of a range of voltages from ±10 mV to ±110 mV in cell pairs that had only one or two active channels. (A) Representative traces of single channel currents in response to ±70 mV pulses in control, Akti, or PIK-75 treated cells. (B-D) Single channel currents of the three treatment conditions were plotted against voltage and values were fitted by linear regression. Unitary conductance (the slope) did not decrease in Akti treated or PIK-75 treated cells, as compared to control; with values of 239 pS, 237 pS, and 224 pS, respectively. The correlation coefficients of all three linear fits were r²=0.99