SMCs were transduced with HIV NL4-3 for 3 h, washed and re-incubated in fresh medium.
Cells and media were harvested at days 1, 3, 6 and 9.
A–B. RNA was extracted and the expression of HIV Gag and APOL1 was detected by RT-PCR.
C. APOL1 concentrations in incubation media were determined with ELISA kit.
The results (mean ± SD) are from three independent sets of experiments carried out in triplicate.
* p < 0.05 vs. control.