A. SMCs were treated with recombinant human IFN-γ for 36 hours at the concentrations indicated (n=3). Protein blots were probed for APOL1 and the same blots were stripped and reprobed for actin.
B. Cumulative densitometric data shown in bar graphs. * p < 0.05 compared with control.
SMCs were treated with 10 ng/ml of recombinant human IFN-γ for the indicated time periods (n=3). Subsequently, cells and incubation media were harvested.
C. Protein blots were probed for APOL1, stripped and reprobed for actin.
D. Cumulative densitometric analysis shown in a bar diagram. * p < 0.05 compared with 0, 12, 48, and 72 h.
E. APOL1 concentrations in incubation media were determined by ELISA. * p < 0.05 compared with control.