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. Author manuscript; available in PMC: 2016 May 15.
Published in final edited form as: Cancer Res. 2015 Mar 25;75(10):2109–2119. doi: 10.1158/0008-5472.CAN-14-3122

Figure 5.

Figure 5

EGFR*-independent glioma cells exist prior to treatment. A, tumor bearing animals grafted with GFP-expressing iEIP glioma cells were switched to Dox and sacrificed at indicated time-points (n = 2 for each). H&E and IHC staining against EGFR or activated caspase3 (Act-Cas3) revealed complete suppression of EGFR* induction at 4-day post treatment and enhanced apoptosis in Dox treated tumors. Scale bars represent 50 μm. B, IF staining revealed a subpopulation of GFP-labeled Ki67-positive proliferative tumor cells persisted through genetic suppression of EGFR* induction. G - GFP; D - DAPI. Scale bars represent 100 μm.