Figure 3. ression of E50K optineurin induced an age-related loss of retinal ganglion cells.
(A) Representative retinal flatmounts from young and aged wildtype (WT) and BAC hOPTNE50K (E50K) mice are shown. RGCs are labeled with the SMI-32 antibody. Magnified regions of retinas are indicated by the white box. RGC axons course toward the center of the retina to exit the eye through the optic nerve. Aged BAC hOPTNE50K retinas exhibited fewer RGCs with smaller somas and thinner axons than WT controls. Scale bar, 500 m, 100 m (high magnification). (B) Quantitative analysis of RGC density showing a decrease in aged BAC hOPTNE50K mice. Data represent means ± SEM, 5 animals per genotype per age group, * p<0.02, ** p < 0.001.
