Figure 4. E50K optineurin is associated with a higher number of damaged axons.
(A) Paraphenylenediamine (PPD) staining in representative optic nerve cross sections from wildtype (WT) and BAC hOPTNE50K (E50K) mice. PPD stains the axoplasm of damaged or dead axons (arrows). Scale bar, 10 μm. (B) Axon counts of healthy axons showed no significant difference between the two genotypes (p = 0.33). However, there was a higher number of damaged or degenerated axons by PPD staining (* p = 0.03) in BAC hOPTNE50K mice (n = 4 animals, 8 optic nerves) than wildtype littermates (n = 6 animals, 11 optic nerves). Data represent means ± SEM.
