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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Dev Biol. 2015 Mar 20;402(1):81–97. doi: 10.1016/j.ydbio.2015.03.007

Fig. 1. Morpholino knockdown causes progressive depletion of Neurod.

Fig. 1

Whole mount immunolabeling for GFP (green) and Neurod (red) protein. Confocal images (merged z-stacks) of the dorsal pancreatic bud in Tg(neurod:EGFP) embryos at 30 hpf. (A, E, I) Control embryos; (E; inset) gray scale. Neurod UTR MO injected specimens, (B, F, J) 2 ng/embryo, (C, G, K) 4 ng/embryo, (D, H, L) 8 ng/embryo. Scale bar = 10 μm.