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. Author manuscript; available in PMC: 2016 Jun 1.
Published in final edited form as: Dev Biol. 2015 Jan 19;402(1):127–141. doi: 10.1016/j.ydbio.2015.01.008

Fig. 6. β-catenin miRNA TP-treated embryos have normal sphincters but less well developed circumesophageal musculature.

Fig. 6

Three day old larvae were immunolabeled with the myosin heavy chain antibody (MHC) (Wessel et al., 1990) to detect circumpharyngeal muscle fibers and sphincters (red arrow-pyloric sphincter; white arrow-anal sphincter) that compartmentalize the larval gut. (A) DIC image of the control larva. (B) Control larva immunolabeled with MHC. (C) Zoomed in view of a normal larval circumpharyngeal muscles. (D) DIC of the β-catenin miRNA TP-treated embryo. (E) β-catenin miRNA TP treated embryo immunolabeled with MHC. (F) Zoomed in view of the larval circumpharyngeal muscles from β-catenin miRNA TP injected larvae. es=esophagus/foregut; st=stomach/midgut; in=intestine/hindgut. (G) The diameter of the circumesophageal muscle fibers is significantly smaller in the β-catenin miRNA TP treated embryos compared to the control embryos.