Table 1.

Primer pairs used to specifically amplify target gene coding for virulence factors (1–9 = toxins; 9–15 fimbriae) of E. coli and 16S rRNA (16-17).

Target gene coding for virulence factors	Oligonucleotide sequences of primers	Reference
(1) LT	F 5′-ATT TAC GGC GTT ACT ATC CTC-3′  R 5′-TTT TGG TCT CGG TCA GAT ATG-3′	[25]
(2) Sta	F 5′-TCC GTG AAA CAA CAT GAC GG-3′  R 5′-ATA ACA TCC AGC ACA GGC AG-3′	[26]
(3) STb	F 5′-GCC TAT GCA TCT ACA CAA TC-3′  R 5′-TGA GAA ATG GAC AAT GTC CG-3′	[26]
(4) Stx1all	F 5′-CGC TGA ATG TCA TTC GCT CTG C-3′  R 5′-CGT GGT ATA GCT ACT GTC ACC-3′	[27]
(5) Stx2all	F 5′-CTT CGG TAT CCT ATT CCC GG-3′  R 5′-CTG CTG TGA CAG TGA CAA AAC GC-3′	[27]
(6) Stx2e	F 5-ATG AAG AAG ATG TTT ATA GCG-3′  R 5′-TCA GTT AAA CTT CAC CTG GGC-3′	[25]
(7) EAST1	F 5′-CCA TCA ACA CAG TAT ATC CGA-3′  R 5′-GGT CGC GAG TGA CGG CTT TGT-3′	[28]
(8) eae	F 5′-GGA ACG GCA GAG GTT AAT CTGCAG-3′  R 5′-GGC GCT CAT CAT AGT CTTTC-3′	[27]
(9) hlyA	F 5′-AGCTGCAAGTGCGGGTCTG-3′  R 5′-TACGGGTTATGCCTGCAAGTTCAC-3′	[29]
(10) F4 (K88)	F 5′-GCT GCA TCT GCT GCA TCT GGTATG G-3′  R 5′-CCA CTG AGT GCT GGTAGT TAC AGC C-3′	[30]
(11) F5 (K99)	F 5′-TGC GAC TAC CAA TGC TTC TG-3′  R 5′-TAT CCA CCA TTA GAC GGA GC-3′	[26]
(12) F6 (P987)	F 5′-TCT GCT CTT AAA GCT ACT GG-3′  R 5′-AAC TCC ACC GTT TGT ATC AG-3′	[25]
(13) F17	F 5′-GGG CTG ACA GAG GAG GTG GGGC-3′  R 5′-CCC GGC GAC AAC TTC ATCACC GG-3′	[30]
(14) F18	F 5′-GTG AAA AGA CTA GTG TTT ATT TC-3′  R 5′-CTT GTA AGT AAC CGC GTA AGC-3′	[31]
(15) F41	F 5′-GAG GGA CTT TCA TCT TTT AG-3′  R 5′-AGT CCA TTC CAT TTA TAG GC-3′	[26]
(16) E16SI	F 5′-CCCCCTGGACGAAGACTCAC-3′  R 5′-ACCGCTGGCAACAAAGGATA -3′	[29]
(17) E16SII	F 5′-AGAGTTTGATGGCTCAG-3′  R 5′-GGACTACCAGGGTATCTAAT-3′	[31]