Table 1.

The sequences of primers and thermal condition used in PCR amplification.

Target genes	Primer used (5′-3′)	Thermal cycling condition	PCR product size
TEM	TAATCAGTGAGGCACCTATCTC GAGTATTCAACATTTCCGTGTC [18, 19]	94°C 3 min → 35 ×  [94°C 30 sec, 45°C 45 sec, 72°C 40 sec] → 72°C 7 min	800 bp
CTX	TTTGCGATGTGCAGTACCAGTAA CGATATCGTTGGTGGCATA [20]	94°C 5 min → 40 ×  [94°C 45 sec, 53.1°C 45 sec, 72°C 1 min] → 72°C 7 min	593 bp
VEB	CGACTTCCATTTCCCGATGC GGACTCTGCAACAAATACGC [9]	93°C 3 min → 40 ×  [93°C 1 min, 54.9°C 1 min, 72°C 1 min] → 72°C 7 min	585 bp
SHV	GGTTATGCGTTATATTCGCC TTAGCGTTGCCAGTGCTC [21]	1 cycle of 5 min at 96°C; 35 cycles of 1 min at 96°C, 1 min at 60°C, 1 min at 72°C; 1 cycle of 10 min at 72°C	867 bp
GES	ATGCGCTTCATTCACGCAC CTATTTGTCCGTGCTCAGG [22]	1 cycle of 5 min at 95°C; 30 cycles of 1 min at 95°C, 45 sec at 55°C, 1 min 30 sec at 72°C; 1 cycle of 8 min at 72°C	846 bp