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Expression of the spi1:EGFP-mCherry/CG2 transgenic fish. (a) Diagram of the spi1:EGFP-mCherry/CG2 construct that contains Tol2 sequences and the cmlc2:GFP expression cassette. (b) Fluorescent images of TG (spi1:EGFP-mCherry/CG2) embryos at 24 or 48 hpf. The white arrowhead indicates spi1 expressing myeloid cells and the yellow arrowhead fluorescence in heart.
