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. Author manuscript; available in PMC: 2015 May 17.
Published in final edited form as: J Cell Physiol. 2011 Aug;226(8):1989–1997. doi: 10.1002/jcp.22533

Figure 3. Immunoprecipitation of SWI/SNF complex core components.

Figure 3

Nuclear proteins from BRG1 mutant cell lines were immunoprecipitated for BRG1 (J1 antibody) of BAF155 (H76, Santa Cruz) and then analyzed by immunoblot for expression of the other core complex members of the SWI/SNF complex. Input samples were run as positive controls. PBS precipitated protein served as a negative control. Protein precipitated with normal rabbit serum (NRS) indicated any background protein precipitated by IgG. HeLa cells were precipitated as a positive control for an intact complex while SW13 serves as a negative control for complex formation because only a minor population expresses BRG1.