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. Author manuscript; available in PMC: 2015 May 17.
Published in final edited form as: J Cell Physiol. 2011 Aug;226(8):1989–1997. doi: 10.1002/jcp.22533

Figure 4. Analysis of RNAi stable clones by protein expression.

Figure 4

Hs578t and HCT116 cells were transfected with shRNAi vectors and selected in growth medium supplemented with puromycin. Total cellular protein was extracted from colonies using urea, separated by SDSPAGE and immunoblotted for BRG1 (G-7, Santa Cruz) in Hs578t cells (A) and HCT116 cells (C) and BRM (Dr. Yaniv, Pasteur Institut) in Hs578t cells (B) and HCT116 cells (D). β-actin served as a loading control.